Dog IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.
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Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
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Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added. Serum may may contain anti-feli
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Estimation of the ratio between antibodies and a peptide : In neutralisation assay use 8,13 l of V-ATPase peptide for 10 ml of reaction volume. In case of any questions please contact support@agrisera
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